双氢青蒿素对肿瘤细胞及肿瘤动物模型的抑制作用

The inhibitory effects of dihydroartennuin on tumor cell and animal remove neoplasia model

目的:观察双氢青蒿素对多种肿瘤细胞和动物移植瘤模型的抑制作用,分析其抗癌作用的特异性。方法:用MTT法、SRB法观察和比较双氢青蒿素对多种肿瘤细胞和正常人体细胞的抑制作用,采用小鼠的B16-BL6、H22和Lewis等肿瘤模型分析双氢青蒿素对肿瘤生长的抑制作用以及铁对双氢青蒿素抗肿瘤效应的药物协同作用。结果:双氢青蒿素对小鼠的B16-BL6、H22和Lewis肿瘤模型的肿瘤生长有较强的抑制作用;提前6h口服少量硫酸亚铁,可明显增强双氢青蒿素对B16-BL6、H22的抗肿瘤效应;双氢青蒿素对K562、KB、HCT、SGC7901、Hela、HepG、BGC823、KCC853、Bel7402、A549、B16-BL6细胞均有抑制作用。IC50(μg/mL)分别为4.12、10.21、11.02、11.77、13.03、18.36、26.47、28.02、44.55、46.28、54.46。在17.5～35μg/mL药物浓度作用下,正常细胞(HELF)有3%～5%生长,而肿瘤细胞没有增长,并有部分死亡。结论:双氢青蒿素对多种肿瘤细胞和动物移植瘤模型具有一定的选择性抑制作用,铁剂有抗癌协同作用。

Objective To obseverve the inhibitory effects of dihydroartennnin on tumor cell and animal neoplasia model. Methods Inhibition effects of dihydroarteannuin on muhi-tumors and normal cells were observed by method of MTT, SRB and animal remove neoplasia model, respectively. The synergistic action of dihydroarteannuin and ferrum on anti-tumors was studied. Results The inhibitive effects of dihydroarteannuin on B16-BL6, H22 and Lewis remove neoplasia model were stronger. Anti-tumor effects of dihydroarteannuin were increasing when mice were taken orally in advance 6 h. Dihydroarteannuin inhibited K562, KB, HCT, SC, C7901, Hela, HepG, B GC823, KCC 853, Be17402, A549, B 16-BL6 cells. IC50 were 4. 12, 10.21,11.02,11.77,13.03,18.36,26. 47,28.02,44. 55,46. 28,54 and 46 μg/mL. Within 17.5 μg/mL to 35 μg/mL concentration, common cells increased 3% ～ 5% , and tumor cells did not and died partly. Conclusion dihydroar-teannuin kills tumor cells and animal remove neoplasia mode specificly, which has synergistic action of anti-tumors with ferrum.