一株产海藻糖合成酶嗜中温芽孢杆菌的鉴定及其酶学性质

Studies on Screening of Trehalose Synthase-Producing Strain and Its Enzymology Properties

由长白山温泉附近土壤中筛选得到产海藻糖舍酶菌株，为革兰氏阳性菌，具有芽孢和鞭毛，其最适生长温度为55℃，对这一菌株进行生理生化指标测定。进一步对该菌株所产海藻糖合酶的酶学性质进行研究，酶反应最适作用pH值为7．0，在pH6．6～7．4时该酶稳定，最适作用温度为35℃，在25-45℃时该酶可稳定保存。

A strain of trehalose synthase-producing thermophilic was isolated from the soil of hot springs of changbai Mountain. The ceils were gram-positive sporulating and motile. The optimum temperature for growth was 55 ℃ ,The trehalose synthase produced by the strain properties were as follows： The optimum pHvalue was 7.0, this enzyme also exhibited a good pH adapt ability and stability,more 80% of the highest level was maintained at the range of pH 6.6-7.4. The optimum reaction temperature was 35 ℃,higher ractivity was maintained at the range of 25-40 ℃.