黄曲霉毒素B_1金标检测体系建立过程中的影响因素

Study on Factors Influencing on the Development of Nanogold-Labeled Assay System of Aflatoxin B_1 in Foods

详细研究了纳米金标记免疫检测体系建立过程中的影响因素,包括检测带的信号强度、封闭试剂,纳米金探针的浓度、包被试剂A和B的浓度、纳米金溶胶的颗粒尺寸,及检测过程中的化学体系等。通过参数优化,确立了建立快速、简易的金标试剂条检测方法的最佳条件。探针溶液中加入保护试剂K后,试剂条的稳定性有明显的提高,常温下试剂条保存期可达120 d(信噪比保留70%以上)。

Effect of the detection line signal, the blocking reagent, the collo.idal gold-labelled probe concentration, the coating reagent A and B concentration, the nanogold size and the chemical system on the development of nangold-labeled immunchromatographic strip （GICA） for analysisi aflatoxin B1 was investigated. The results showed that, the analysis time of aflatoxin B1 only 10 min with the optimal conditions.