益生菌Lactobacillus salivarius的培养条件

Study on Cultural Conditions of Probiotic Lactobacillus salivarius

Lactobacillus salivarius是来自人体肠道的一株重要的益生菌。作者对其培养条件进行了研究。确定了最佳培养温度为37℃和培养基起始pH值为6.4。当在改良的MRS培养基中添加质量浓度为10 g/L碳酸钙,并经过一次中间补糖(8 g/L)培养,菌体干重达7.12 g/L活菌数为3.89×108cfu/mL。

Lactobacillus salivarius is one of the most important bacterium having health-promoting effects in the human intestinal tract. In this study, the optimum conditions for L. salivarius growth were investigated. The optimum temperature and initial pH was 37 ℃ and 6.4, respectively, when 10 g/L Calcium carbonate was feed to the modified MRS cultural medium （20 g/L glucose,5 g/L peptone, 20 g/L beef extract）, and 8 g/L glucose was feed at 14 h, the biomass was achieved at 7. 12 g/L. The living cell number was 3.89× 10^8 cfu/mL.