美味牛肝菌胞外多糖高产菌株的诱变选育

Screening of High Exopolysaccharide Yield Strain of Boletus edulis by UV Ray

对美味牛肝菌胞外多糖的高产菌株进行了诱变选育。结果表明，美味牛肝菌原生质体制备的最佳条件是56h菌龄的菌丝体，酶解温度31-35℃，酶解时间2h，2％纤维素酶和蜗牛酶体积比1：1，稳渗荆0．01092g／mL的甘露醇；原生质体产量是3．71×10^5个/mL，再生率是7．32％；15w紫外灯30cm处照射时间6min，对原生质体进行诱变处理。选育出一株胞外多糖产量比原始菌株高30．40％的菌株。

To enhance the exopolysaccharide production by Boletus edulis, the strain was mutagenized by using UV-irradiation was performed, and the conditions for it s protoplast preparation and regeneration of the mycelium incubation time, enzyme concentration, enzyme temperature, enzyme time, and osmotic stabilizer were examined. The result s showed that the mycelia incubated for 56 h were most suitable for protoplast release. The mixture solution of 1% fiber enzyme/1% snail enzyme and 0. 010 92 g/mL mannitol was the most effective osmotic stabilizer and was most favorable for protoplast preparation. The suitable incubation temperature and time for the release of protoplast was 30-35 ℃ and 2 h. With the optimum conditions, the protoplasts and the regeneration rate was 3.71 × 10^5 and 7. 32%, respectively. Compared with that of parent strain, the yield of exopolysaccharide production by mutant strains increase 30. 4%.