碳、氮源对PVA降解混合体系降解能力的影响及PVA降解机理初探

Effect of Different Carbon and Nitrogen Sources on PVA degradation in a Mixed Culture and PVA Degradation Mechanism

以能完全降解1 g/L PVA的一个混合体系为研究对象,研究了碳、氮源对该混合体系降解PVA的影响。实验表明,补充有机氮源有利于混合体系菌体的生长,并且能提高混合体系对PVA的降解能力。进一步的研究发现,其它碳源的补充有利于菌体的生长,但对混合体系降解PVA产生一定的抑制作用。根据初步研究结果推断,该混合体系所产的PVA降解酶主要结合在细胞膜上,部分PVA进入细胞后被降解。

The effect of different concentration of carbon and nitrogen sources was investigated in a mixed system. The results indicated that the addition of organic nitrogen source was not only advantageous to the growth of the mixed culture, but also propitious to PVA degradation. Further results indicated that the presence of other carbon sources, though was also advantageous to cell growth, inhibited PVA degradation. By analyzing the existence of residual PVA in cells, PVA-degrading enzymes was assumed to mostly linked with cell membrane and partial PVA degradation after entering into cells.