植物乳杆菌HO-69产抗菌肽的培养条件

Study on Bacteriocin Production Condition of Lactobacillus Plantarum HO-69

对L.plantarumHO-69产抗菌肽的营养和培养条件进行了研究。实验结果表明,MRS培养基是其产抗菌肽的最适培养体系。以碳源、氮源、缓冲盐等为研究因子,对MRS设计部分因子重复实验,采用回归分析确定K2HPO4和牛肉膏为抗菌肽产生的显著影响因子,综合考虑抗菌肽活力与纯化的难度,确定K2HPO4与牛肉膏的质量浓度分别为12 g/L与16 g/L,蛋白胨质量浓度为6 g/L。响应面分析确定HO-69产抗菌肽的最适培养条件为:起始pH值6.61,36.12℃发酵13.87 h,在此条件下发酵液的效价由80 AU/mL提高到320 AU/mL,抗菌肽的产量增加为原来的4倍。

The culture conditions of Lactobacillus plantarum HO-69 producing antipeptide were studied. MRS broth was the suitable media. Influence of carbon, nitrogen source and buffer on antipeptide production was evaluated using a fractional factorial design. Regression analysis showed that dipotassium hydrogen phosphate and beef extract plays key role on antipeptide production. The optimal concentrations of K2HPO4, beef extract and peptone were 12 g/L , 16 g/L and 6 g/L, respectively. The optimum fermentation parameters from Response Surface Methodological experiment were：13.87 h fermentation time, culture temperature of 36.12℃ and an initial pH of 6. 61. The optimized medium and fermentation condition allowed antipeptide production to be increased from 80 AU/ mL to 320 AU/ mL, yield 400% times of original conditions.