与黄瓜雌性性状连锁的cs-acs1g基因特异片段克隆与鉴定

Cloning and Identification of Special cs-acs1g Gene-Linked to Gynoecious in Cucumber

目的快速、准确划分出黄瓜雌性系是蔬菜育种工作者面临的重大任务,为了弥补目前物理方法鉴定的缺陷,本试验开展分子水平上鉴定的研究。方法从GenBank中查找到cs-acs1g基因序列,根据该序列设计两对引物,然后从黄瓜(C0208)雌性系材料中克隆2个基因片段,利用PCR扩增方法及田间调查手段相结合,验证cs-acs1g基因的2个片段和雌性系之间的相互关系。结果cs-acs1g基因的1013bp片段为黄瓜(CucumissativusL.)雌性系所特有,非雌性系没有此片段,而cs-acs1g基因的540bp片段无品种特异性。结论利用雌性系特异基因进行植株鉴定可以应用到实践中,是一种简便、快捷的可取方法。

[ Objective ] This study was conducted to help identify gynoecium quickly and.effectively. Our target was to develop a new method at the molecular level to overcome physical shortcomings. [ Method ]We discovered the sequence of cs-acs1g gene from GenBank and designed two pairs of primers according to this gene. Two gene partials were cloned from gynoecium of cucumber （C0208）. The relationship between cs-acslg gene and gynoecium was validated through a combination of PCR method and investigations.[Result] 1 013 bp partial ofcs-acslg gene was unique to gynoeeium of cucumber （Cucumis sativus L.） while this phenomenon didn＇t exist in gynoecium, 540 bp partial of this gene existed commonly in all the cucumber varieties. [ Conclusion ]It was an available measure depending on gynoeeium＇s special gene and enabled quick identification.