水溶性灵芝肽在动物体外的抗氧化活性

The Antioxidative Activity of Water-Soluble Peptides from Ganoderma lucidum in vitro Test

目的研究水溶性灵芝肽在动物体外的抗氧化活性。方法用丙二醛(MDA)试剂盒测定MDA含量,用分光光度法测定大鼠血清自氧化溶血及小鼠肝线粒体肿胀度。结果当水溶性灵芝肽的剂量为0.25mg·ml-1时,对大鼠红细胞自氧化溶血的抑制率达62.33%,对溶血过程中MDA生成的抑制率达91.24%;在有或无自由基诱导剂(Fe2+或H2O2)时,0.35mg·ml-1的水溶性灵芝肽均能极显著抑制小鼠肝匀浆中MDA的生成(P<0.01),且抑制率大致相当,为60%左右;当水溶性灵芝肽剂量为1.00mg·ml-1时,对线粒体肿胀度的抑制率为90.52%,对线粒体中MDA生成的抑制率达65.42%,且呈明显剂量-效应关系。结论水溶性灵芝肽在体外具有明显的抗氧化作用。

[ Objective ] The antioxidative activity of water-soluble peptides from Ganoderma lucidum （GLP） in vitro test was studied. [Method] The reagent kits were used to determine the content of malondialdehyde （MDA）. The auto-hemolysis of red blood cells （RBC） in rats and the swelling of rats liver mitochondria were observed by means ofspectrophotometer. [Result] The inhibiting rate of 0.25 mg·ml^-1 GLP on the auto-hemolysis of rats RBC was 62.33%. The inhibiting rate on the MDA formation in rat RBC was 91.24%. 0.35 mg ml^-1 GLP. It could prevent the MDA formation in mice liver homogenate （P〈0.01） with or without free radical inducers （Fe^2＋or H2O2）. They had approximate inhibiting rates around 60%. When 1.00 mg·ml^-1 GLP was added, the inhibiting rate on the swelling of mice liver mitochondria was 90.52%. The inhibiting rate on the MDA formation in mice liver mitochondria was 65.42% in a dose-dependent manner. [ Conclusion ] GLP was a good antioxidant in vitro test.