外源NO供体对梨叶片感染轮纹病菌后叶绿素及其荧光参数的影响

Effect of exogenous NO donor on chlorophyll of pear leaves that are infected with pnysalosproa piricola nose and fluorescence parameters

用0.3mmol.L-1NO供体硝普钠(Sod ium n itroprusside,SNP)预先处理梨叶片,3d后接种纶纹病菌(Physalospora P iricola Nose),8d后开始测定各种指标.结果表明,浓度为0.3mmo1.L-1的SNP处理,能提高梨病叶中的叶绿素a、叶绿素b、总叶绿素和类胡萝卜素的含量以及原初光能转换效率(Fv/Fm)、PSⅡ潜在活性(Fv/Fo)、光合电子传递量子效率(φPSⅡ)和光化学猝灭系数(qP)的值.能抑制非光化学猝灭系数(qN)的值.可初步确定,较低浓度的SNP可缓解轮纹病菌对梨叶片的伤害作用.

After pear leave are infected for five days, use SNP in different density to deal with in order to search a SNP density that can most effectively relieve harm to the pear leave. The result shows that using SNP in the density of 0.3 mmol·L^-1 to treat the diseased leave can raise chlorophyll a and chloriophyll b, total chlorophyll, carotenoid, maximal photochemical efficiency of PS Ⅱ （ Fv/Fm ）, potential activity of PS Ⅱ （ Fv/ Fo） ,actual PS Ⅱ effciency （ ФPS Ⅱ ）, and photochemical quenching （ q^P ） value, and reduce non photochemical quenching （q^N） value, while using the density of 0.1 and 0.6 mmol ·L^-1 to treat diseased pear leave, its effects on chlorophyll, carotenoid and chlorophyll fluorescence parameters are not apparent. In the treating group of SNP in the same density , with the prolonging of the treating time, effects on them will vary in different degree. It can be initially confirmed that SNP in a relatively low density can palliate harm to pear leave after they are infected with Pnysalospma Prrieola Nose.