多发性硬化患者外周血记忆性T细胞亚群的研究

Study on memory T cell subsets in peripheral blood of patients with multiple sclerosis

目的:近来研究表明中心记忆性和效应记忆性T细胞构成记忆性T细胞的两个亚群,但在多发性硬化(Multi-plesclerosis,MS)中的作用尚不清楚,因此测定了MS患者外周血记忆性T细胞亚群的水平,并进一步探讨了可能导致记忆性T细胞亚群变化的机制。方法:采用流式细胞仪和酶联免疫吸附分析(Enzyme-linkedimmunosorbentassay,ELISA)分别检测未治疗MS、正常对照组外周血记忆性T细胞亚群的阳性率和血浆IL-15浓度。进一步根据MS临床表现分为复发-缓解型MS(Relapse-remissionMS,RRMS)和慢性进展型MS(ChronicprogressiveMS,CPMS)两个亚组。结果:与正常对照比较,在CD8+T细胞亚群中,MS患者中心记忆性T细胞(CentralmemoryTcell,TCM)、终末效应记忆性T细胞明显增多和减少(P<0·05和P<0·01),RRMS患者终末效应记忆性T细胞明显少于正常对照(P<0·05);MS患者血浆IL-15水平较正常对照明显升高(P<0·05)。结论:研究显示了MS患者中CD8+TCM上调,可能反映了在MS早期被诱导产生的一个持续慢性炎性应答,我们推测CD8+TCM的异常改变可能在维持MS慢性炎症的过程中起着重要作用,而IL-15则可能参与了促进TCM分化的调节过程。

Objective： To explored the mechanisms that lead to the changes of the memory T cell subsets. Methods： By using of flow cytometry and enzyme-linked immunosorbent assay（ ELISA）, we detected the percentage of memory T cell subsets and plasma concentration of interleuldn-15 （ IL-15 ） in peripheral blood of MS patients and healthy individuals. Furthermore, MS patients were subdivided into both relapse-remission MS（RRMS） and chronic progressive MS（CPMS） group according to the clinical features. Results： Compared to healthy controls, there was an increase and decrease in CD8^＋T CM and terminal effector memory CD8^＋T cell in MS patients（P 〈 0. 05 and P 〈 0. 01 ）. After the clinical subdivision, the RRMS patients showed an increased terminal effector memory CD8^＋T cell compared with healthy controls（P 〈0. 05）. In addition, MS patients showed a higher level of plasma IL-15 than these of control group （P 〈 0. 05 ）. Conclusion：The upregulated CD8^＋T CM in MS patients may reflect a persistent chronic inflammatory response that may have been induced during early stages of the disease. We speculate that this derangement may play an important role in the maintenance of chronic inflammation, while IL-15 may participate in the immunoregulatory process of promoting TCM differentiation.