精神分裂症IL-1β、TNF-α和酪氨酸羟化酶的基因表达水平

The gene expression levels of IL-1β,TNF-α and tyrosine hydroxylase in peripheral blood mononuclear cell of schizophrenic

目的:检测精神分裂症患者IL-1β、TNF-α和酪氨酸羟化酶(TH)的基因表达水平,探讨精神分裂症的外周神经免疫机制。方法:采用RT-PCR和半定量技术,分别检测39例精神分裂症患者、25例同胞对照和30例正常对照外周血单个核细胞IL-1β、TNF-α和TH的基因表达水平。结果:研究显示IL-1β在病例组、同胞对照组和正常对照组的基因表达水平分别为1·52±1·01、1·18±0·99和0·55±0·33;TNF-α在三组样本的基因表达水平分别为1·52±1·09、1·01±0·87和0·61±0·32;TH在三组样本的基因表达水平分别为0·74±0·38、0·70±0·29和0·28±0·20。其中病例组与同胞对照组IL-1β、TNF-α、TH基因表达水平无显著差异(P>0·05);病例组和同胞对照组的IL-1β、TNF-α、TH基因表达水平均显著高于正常对照组(P<0·05或P<0·01),且对照组IL-1β和TNF-α基因表达水平显著相关(r=0·847,P<0·01);IL-1β和TH基因表达水平显著相关(r=0·666,P<0·01)。病例组只有IL-1β和TNF-α基因表达水平表现为显著相关(r=0·942,P<0·01)。结论:精神分裂症患者可能存在致炎性细胞因子和儿茶酚胺类神经递质的过度表达,且可能受遗传背景影响。儿茶酚胺类神经递质和致炎性细胞因子的基因表达之间可能具有一种交互机制,而精神分裂症患者的这种交互机制发生了紊乱。

Objective：To investigate the gene expression levels of IL-1β, tumour necrosis factor-or and tyresine hydroxylase of schizophrenic, and explore the neuroimmunology mechanism of schizophrenia. Methods：The gene expression levels of IL-1β, TNF-α and TH in the peripheral blood mononuelear cell of 39 schizophrenic patients were measured with RT-PCR and semi-quantification technique, together with 25 sibling controls and 30 normal controls. Results： The gene expression levels of IL-1β were 1.52 ± 1.01 in schizophrenic, 1.18 ±0. 99 in sibling controls and 0. 55 ±0. 33 in normal controls; the gene expression levels of TNF-α were 1.52 ± 1.09 in schizophrenic, 1.01 ± 0. 87 in sibling controls and 0. 61 ± 0. 32 in normal controls; the gene expression levels of TH were 0. 74 ± 0. 38 in schizophrenic, 0. 70 ± 0. 29 in sibling controls and 0. 28 ± 0. 20 in normal controls. No significant difference in each gene expression level was found between schizophrenic and siblings（ P 〉 0. 05 ）, but each gene expression level was higher in schizophrenic or siblings than in normal controls （ P 〈 0. 01 or 0. 05）. The correlation between the gene expression levels of IL-1β and TH were found in controls （ r = 0. 666, P 〈 0. 01 ）, not in schizophrenic （ P 〉 0. 05 ）, and the correlation between the gene expression levels of IL-1β and TNF-α were significant in all groups（ r = 0. 847 or 0. 942, P 〈 0.01 ）. Conclusion： Prointlammation eytokines and catecholamines have been overexpression in the peripheral blood mononuelear cell of schizophrenic. Hereditary factor maybe play a very important role in the regulation of gene expression levels. And the correlation between Catecholamines and proinflammation eytokines, which exists in the controls, is broken in schizophrenic patients.