乙型肝炎患者血清sICAM-1、sVCAM-1含量的检测及其意义

Serum level of soluble intercellular adhesion molecule-1(sICAM-1) and soluble vascular cell adhesion molecular-1(sVCAM-1) and its clinical significance in patients with viral hepatitis B

目的:通过观察乙型肝炎患者血清中sICAM-1和sVCAM-1的水平及其与ALT、AST相关性,探讨Ⅰ型超敏反应与乙型肝炎发病机制的关系。方法:应用酶联免疫吸附实验,检测45例乙型肝炎患者及15例对照组血清sICAM-1和sV-CAM-1水平;应用全自动生化分析仪检测患者血清ALT和AST水平,并观察与sICAM-1和sVCAM-1的相关性。结果:①7例急性肝炎患者血清中sICAM-1和sVCAM-1含量明显高于正常对照组;②38例慢性肝炎患者血清中sICAM-1和sVCAM-1含量也明显高于正常对照组;③13例中度慢性肝炎患者的sICAM-1和sVCAM-1含量显著高于16例轻度慢性肝炎患者;④9例重度慢性肝炎患者的sICAM-1和sVCAM-1含量也明显高于轻度组和中度组;⑤sICAM-1水平与血清内ALT、AST含量呈显著正相关;⑥sVCAM-1水平与血清ALT、AST含量也呈显著正相关。总之,在以上几组中,急性乙型肝炎组的sICAM-1和sVCAM-1的升高最显著,并依次为慢性肝炎重度组、中度组和轻度组。结论:①急、慢性乙型肝炎患者血清中sICAM-1和sVCAM-1水平可反映肝损害程度;②检测急、慢性乙型肝炎患者血清sICAM-1和sVCAM-1水平,对判断患者的病情和预后有一定的参考价值;③作为Ⅰ型超敏反应性炎症的重要指标,sICAM-1和sVCAM-1可能参与了乙型肝炎的发病和肝细胞的免疫损伤过程。

Objective：To further explore the relationship between Type I hypersensitivity reaction＋and the mechanism of the viral hepatitis B through the research of soluble intercellular adhesion molecular-1 （ sICAM-1 ） and soluble vascular cell adhesion molecular-1 （ sVCAM-1 ） levels in serum in patients with viral hepatitis B related tO Type I hypersensitivity reaction and the damage of liver cells , and therefore, provide new theory for the perfection of the immunological mechanism of hepatitis B, especially.acute hepatitis B. Methods：Serum slCAM-1 ar/d sVCAM-1 levels were measured in 45 patients with viral hepatitis B and 15＋ normal cases using double antibody sandwich ELISA method. ALT and ：AST levels were studied using omni autonaatic biochemistry analyzer and its correlation with slCAM-1 and sVCAM-1 were observed. Results： （1）The slCAM-1 and sVCAM-1 levels of 7 acute hepatitis B were significantly higher than that of the normal cases. （2）The slCAM-1 and sVCAM-1 levels of 38 chronic hepatitis B ＋were also significantly ：higher than that of He normal cases. （3）The sICAM-1 and sVCAM-1 levels of 13 cases moderate chronic hepatitis B were significantly higher than that of 16 cases mild chronic hepatitis B. （4）The sICAM-1 and sVCAM-1 levels of 9 serious chronic hepatitis B were signiticanfly higher than that of the group of mild chronic hepatitis B. （5）Levels of slCAM-1 were significantly positively correlated with serum ALl＂ and AST. （6） sVACM-1 levels were significantly positively correlated with serum ALT and AST. In conclusion, slCAM-1 and sVCAM-1 levels in patients with acute hepatitis B were most remarkably increased, and then serious chronic hepatitis B, moderate chronic hepatitis B, light chronic hepatitis Bin Sequence. Conclusion：（1）Serum slCAM-1 and sVCAM-1 levels in patients with viral hepatitis B＇ may reflect the damage of liver. （2）Examination of serum SlCAM-1 and sVCAM-1 levels in patients with hepatitis B can be used to judge the patient＇ s condition and to diagnose. （3）As an important indication of the inflammatory in Type I hypersensitivity reaction, sICAM-1 and sVCAM-1 may interfere the appearence of hepatitis B and the procedure of immunological damage of liver cells.