肺表面活性物质治疗豚鼠分泌性中耳炎的疗效观察

Effects of ectogenic surfactant on SOM in Guinea pigs

目的研究外源性表面活性物质治疗豚鼠分泌性中耳炎的效果,寻找分泌性中耳炎新的治疗方法。方法将分泌性中耳炎豚鼠动物模型随机分为A、B、C 3组,每组各6只(12耳),分别用外源性表面活性物质、地塞米松和生理盐水注入豚鼠中耳腔内,然后观察豚鼠中耳变化;分析豚鼠咽鼓管内表面活性物质的生化成分和活性变化。结果A组的磷脂酰胆碱(PC)和磷脂酰乙醇胺(PE)分别为(23.3±2.2)%和(42.5±3.6)%,B组分别为(17.0±1.9)%和(36.7±3.1)%,C组分别为(15.8±1.2)%和(35.8±3.1)%。A组的最低表面张力(γmin)为(11.8±2.3)毫牛顿/米(mN/m),B组为(20.2±2.7)毫牛顿/米(mN/m),C组为(19.4±2.6)毫牛顿/米(mN/m)(P<0.001)。结论咽鼓管内表面活性物质的异常是引起咽鼓管功能障碍的重要原因之一,外源性表面活性物质对分泌性中耳炎有一定疗效。

Objective To study the effects of ectogenie surfaetant on secretory otitis media （SOM） in guinea pigs and explore a new treatment method for SOM. Methods Guinea pigs suffering from SOM were divided into three groups randomly, 6 for each group. Ectogenic surfactant, dexamethasone and 0. 9% sodium chloride solution were injected into guiea pigs＂ tympanic cavities of group A, B and C respectively. The biochemical components and activity change of surfaetant of Eustachian tube were analysed. Results Phosphatidyleholine （PC） and phosphatidylethanolamina （PE） were （23. 3± 2. 2）%, （42. 5± 3. 6）% in group A, （17. 0± 1.9）%, （36. 7± 3. 1） % in group B and （15. 8± 1.2） %, （35. 8± 3. 1） % in group C respectively. The minimal surface tensions （Trnin） of group A, B and C were （11.8＋2. 3） mN/m, （20. 2±2. 7） mN/m and （19.4±2. 6） mN/m （ P 〈 0. 001）. Conclusion The abnormality of surfactant of Eustachian tube is an important cause resulting in functional disorder of Eustachian tube. Ectogenic surfactant is effective for the treatment of SOM.