白英诱导人肺癌细胞凋亡及对Bcl-2的影响

Apoptosis of Human Lung Cancer Cell Line SPC-A-1 Induced by Solanum Dulcamara Water Extract

目的:研究白英水提取物对人肺腺癌SPC-A-1细胞株的凋亡诱导作用及对其Bcl-2表达的影响;探讨白英提取物诱导SPC-A-1细胞凋亡的可能机制。方法:选用人肺腺癌SPC-A-1细胞株为研究对象,在体外与白英提取物4种不同浓度共同培养24、48、72、96h,采用MTT法检测白英提取物对SPC-A-1细胞增殖活性的影响;琼脂糖凝胶电泳、流式细胞仪检测白英水提取物处理的SPC-A-1细胞株细胞凋亡;比较白英提取物处理前后SPC-A-1细胞凋亡相关基因Bcl-2表达的变化。结果:白英提取物抑制SPC-A-1细胞增殖活性;琼脂糖凝胶电泳可见凋亡梯带;SPC-A-1细胞有很低的自然凋亡率(1.37±0.52)%,50.0mg/ml白英提取物作用SPC-A-1细胞48h后,凋亡率为(33.25±4.39)%(P<0.05);50.0mg/ml白英提取物处理48h后的细胞凋亡相关基因Bcl-2表达率(24.41±2.96)%比未经处理的细胞(53.53±4.80)%表达率低(P<0.001)。结论:白英水提取物对人肺腺癌SPC-A-1细胞株有抑制增殖、诱导其凋亡作用,细胞凋亡的机制可能与细胞凋亡相关基因Bcl-2表达下调有关。

Objective：To study the effect of solanum dulcamara water extraet on a human lung cancer cell line and to examine its possible mechanism.Methods：The human lung caneer cell line SPC-A-1 was in vitro co-eultured with solanum duleamara at 4 different concentations for 24.48,72 and 96h;the MTT assay was used to determine the effect of solanum dueamara water extraet on SPC-A-1 cell proliferation;agarose gel electrophonesi（AGE）and flow eytometry were used to detect the apoptosis rate of SPC-A-1 cells exposed to the solanum duleamara water extract and the change in expression of the apoptosis related gene bcl-2 gene,before and after exposure to the solanum duleamara.Results：The solanum duleamara water extract inhibited prliferation of SPC-A-1 cells ,and an apoptotic ladder was deteeted using AGE.There was a very low rate of natural apoptosis（1.37%±0.52）,After exposing the SPC-A-1 cells for 48h to 50.0mg/ml solanum dulcamara extract.the apoptosis rate was 33.25%±4.39（P〈0.05）,The expression of the apoptosis related gene bcl-2 after treatment with 50.0ng/ml solanum dulcamara extract for 48h（24.14%±2.96）was lower compared to untreated cells（53.53%±4.80）（P〈0.001）.Conclusion：A specific concentration of solanum dulcamara water extract can inhibi SPC-A-1 cell proliferation and can induce apoptosis of SPC-A-1 cells,The mechanism of action is related to expression of the bcl-2 gene.