蛹虫草液体的深层发酵

Submerged fermentation of Cordyceps militaris

以菌丝体质量浓度(干重)和发酵液中甘露醇浓度为指标,研究温度、初始pH值、培养时间等因素对蛹虫草液体培养的影响,得到蛹虫草摇瓶液体培养的最佳温度为25.0℃,初始pH值为6.10,培养时间为5 d;蛹虫草液体发酵最适培养基的组成为:20 g/L蔗糖+5 g/L蛋白胨+1 g/L MgSO4.7H2O。在此基础上进行小型分批式发酵罐实验,研究有利于蛹虫草生长及活性物质生成的pH控制策略。研究结果表明,pH分段控制的液体深层发酵对蛹虫草生长最为有利,菌丝体质量浓度为17.31 g/L,甘露醇质量浓度达43.47 g/L。

The influences of culture conditions such as temperature, initial pH value and cultivation time on biomass and the production of mannitol of Cordyceps rnilitaris were investigated. The optimal conditions were obtained as follows： 25.0 %, pH 6.10 and cultivation time of 5 d. The optimum medium in submerged fermentation for Cordyceps militaris was also investigated. The optional medium composition is as follows： 20 g/L sucrose＋5 g/L peptone＋1g/L MgSO4·7H2O. In addition, the batch fermentation in a 12L fermentation tank was used to study the effect of pH value. The results show that a two-stage pH control strategy is best for biomass and mannitol production in the batch fermentation of Cordyceps militaris. The concentrations of biomass and mannitol in the solution reach 17.31 g/L and 43.47 g/L, respectively.