葛根素预处理与缺血预处理对大鼠心肌缺血再灌注损伤的保护作用

The Protective Effect of Puerarin Preconditioning against Myocardial Ischemia-reperfusion Injury in Rats

目的:观察葛根素(PUE)预处理与缺血预处理(IPC)对大鼠心肌缺血再灌注损伤(I/R)的保护作用,以进一步明确PUE药物预处理(PPC)的心肌保护效应。方法:采用开胸结扎冠状动脉左前降支的方法复制大鼠心肌I/R模型,设立假手术组(sham组)、I/R组、IPC组、PUE组。用红四氮唑染色法测定各组心肌梗死面积,TUNEL法检测心肌细胞凋亡情况,同时测定血清肌酸激酶(CK)活力和血清肌钙蛋白T(TnT)水平。结果:I/R组心肌梗死面积、血清CK活力、TnT浓度以及心肌细胞凋亡指数较sham组显著增高(P<0·05或P<0·01),与I/R组比较,PUE组和IPC组心肌梗死面积明显缩小(P<0·05),血清CK活力、TnT浓度(P<0·05或P<0·01)、心肌细胞凋亡指数显著降低(P<0·01),PUE组与IPC组间各指标差异无明显性(P>0·05)。结论:PUE预处理明显减轻了大鼠心肌I/R,并与IPC的保护效应相似,产生了PPC效应。

Objective： To investigate whether puerarin preconditioning has .protective effect against myocardial ischemia-repeffusion injury in rats compared with ischemic preconditioning. Methods： Rat heart ischemia-reperfusion injury （I/R） was induced by occluding the left anterior descending coronary artery for 45 minutes and repeffusion for 120 minutes. Fourty-four rats were randomly divided into four groups （ 11 in each）： （ 1 ） Received sham operation （ group sham） ; （2） I/R model only （ group I/R） （ 3 ） ischemic preconditioning （ group IPC, 3 times of ischemia 5 min and repeffusion 5 min prior to I/ R）, and （4） puerarin （PUE） pretreated group （group PUE, 100mg/kg of PUE were intravenously injected 5 minutes before ischemia） . TIC staining method was used to determine the myocardial infarct size. Cardiac myocytes apoptosis was detected by TUNEL. The activity of creatine kinase （CK） and concentration of troponin T （TnT） in serum were measured simultaneously. Results： Myocardial infarct size, cardiomyocytes apoptosis index, the activity of CK and concentration of TnT in serum of group I/R were obviously higher than those in group sham （P 〈 0.05 or P 〈 0.01 ）. Compared with group I/R, all these parameters were significantly reduced in IPC and PUE pretreated groups （P 〈 0. 05 or P 〈0. 01 ）. Furthermore, there was no significant difference between IPC and puerarin pretreated groups in all these parameters （P 〉 0.05 ）. Conclusions： Puerarin preconditioning （ administrating puerarin before ischemia） can significantly reduce myocardial ischemia-repeffusion injury in rats, and its proctective effect is similar to that of IPC.