葛根素对大鼠脑缺血再灌注损伤后Fas蛋白表达的影响

Effect of puerarin on the expressions of fas in local cerebral ischemia and reperfusion injury in rat

目的探讨葛根素对大鼠脑缺血再灌注损伤后Fas蛋白表达的影响。方法采用闭夹大脑中动脉后再通造成局部脑缺血再灌注模型,免疫组化SP法测定Fas表达情况,光镜观察脑组织Fas蛋白阳性细胞数和死亡细胞数。结果正常对照组Fas阳性细胞数及死亡细胞数明显少于再灌注组,再灌注葛根素组死亡细胞数和Fas阳性细胞数明显少于再灌注对照组(P均<0.01)。结论葛根素对大鼠急性脑缺血再灌注损伤具有保护作用。

OBJECTIVE To study the effect of puerarin on the expression of Fas in local cerebral ischemia and repeffusion injury in rat. METHODS Rat model of cerebral ischemia and ischemia-repeffusion was established with ligated middle cerebral artery. The Fas expression was detected by SP immunohistochemical technique, the histopathological change was assessed with microscope in the rats with local cerebral ischemia and ischemia-reperfusion injury. RESULTS The Fas positive cells and the number of death cells were significantly less in the normal groups than those in the ischemia-reperfusion ones. Of the ischemia-reperfusion groups, the number of Fas positive cells and death cells were significantly less in puerarin groups than those in control groups. CONCLUSION Puerarin can attenuate the tissue injury caused by local cerebral ischemia and ischemia-reperfusion,its protected mechanism may be different according to the injury mechanism.