大鼠肢体缺血再灌注后肝细胞损伤及牛磺酸的保护效应

The effect of taurine on the liver function after limb ischemia-reperfusion in rats

目的观察大鼠肢体缺血再灌注后肝脏的损伤性变化,以及牛磺酸(taurine)对肝脏损伤性变化的影响,探讨牛磺酸对大鼠肢体缺血再灌注后肝脏功能保护作用及可能机制。方法实验用W istar大鼠30只,随机分为对照(control)组,缺血再灌注(ischem ia reperfusion,IR)组和牛磺酸+缺血再灌注(taurine+Ischem ia reperfusion,TR)组(n=10)。分别测定各组动物肝组织黄嘌呤氧化酶(XOD)、丙二醛(MDA)、活性氧(ROS)、髓过氧化物酶(MPO)、Ca2+含量和肝细胞线粒体琥珀酸脱氢酶(SDH),Na+-K+-ATPase,Ca2+-Mg2+-ATPase,Ca2+含量的变化。结果发现大鼠肢体缺血再灌注后肝脏功能明显受损,牛磺酸减轻了由于肢体缺血再灌注引起的肝细胞和线粒体的钙超载及过氧化等损伤。结论牛磺酸可以避免或减轻大鼠肢体缺血再灌注继发的肝脏损伤。

OBJECTIVE To observe the effect of taurine on the liver function after limb isehemia-reperfusion in rats. METH- ODS 30 Wistar rats were randomly divided into three groups, including control group,isehemia reperfusion（IR） group and taurine ＋ isehemia reperfusion （TR） group（ n = 10）, The levels of XOD, MDA, ROS, MPO, Ca^2＋ in liver tissues and the levels of Ca^2＋ ,SDH, Na^＋ -K^＋ -ATPase, Ca^2＋ -Mg^2＋ -ATPase in liver mitoehondria were measured respectively. RESULTS Compared with the IR group, the levels of XOD, MDA, ROS, MPO, Ca^2＋ in liver tissues and the levels of Ca^2＋ in liver mitechondria were decreased in TR group. The activity of SDH, Na^＋ -K^＋ -ATPase, Ca^2＋ -Mg^2＋ -ATPase in liver mitoehondria was improved by taurine. CONCLUSION The results indicated that the taurine can protect liver function from limb isehemia-reperfusion injury in rats.