广地龙中琥珀酸的毛细管电泳电导法测定

Determination of succinic acid in Pheretima aspergillum by capillary electrophoresis with a conductivity detector

目的 建立毛细管电泳电导法测定广地龙中琥珀酸含量的方法。方法 以1．0mmoL／L Tris-10mmoL／L H3BP3-0．5％四乙烯五胺-0．025mmol／L十六烷基三甲基溴化铵为分离介质，使用未涂层融硅毛细管柱（50cm×75μm），分离电压-10kV，电导检测器检测。结果 琥珀酸的线性范围5．00～80．0μg/mL（r=0．9998），平均加样回收率98．4％，RSD=2．2％。结论 该法操作简便，快速。

OBJECTIVE A capillary electrophoresis with conductivity detection method to determine succinic acid in Pheretima aspergillum was described. METHODS A fused-silica capillary tube（50cm×75 μm）was used, at the separation potential of -10kV succinic acid was detected directly in running medium of 1.0 mmol/L riffs 10 mmol/L H3 BO3-0.5% tetren-0. 025 mmol/L CTAB（ pH = 11.45）. RESULTS The linear range of succinic acid was 5.00μg/mL - 80.0μg/mL（ r =0.999 8）, the average recovery of added sample was 98.4% with the RSD = 2.2%. CONCLUSION This method is simple and quick.