白藜芦醇对卵巢切除大鼠动脉血压及升压反应的影响(英文)

Effects of resveratrol on arterial blood pressure and pressor response in ovariectomized rats

选用雌性Wistar大鼠,体重214．07±4．85 g,于双侧卵巢切除后分别皮下注射白藜芦醇RES(0．4 mg／kg)和17β-雌二醇EST(1 mg／kg)或等量溶媒芝麻油,以假手术大鼠作为对照．处理21天后,观察去势鼠及。RES或EST替代治疗大鼠对血管紧张素Ⅱ、去甲肾上腺素、肾上腺素的升压反应以及压力感受性反射敏感性的变化．结果发现:去势大鼠收缩压(SP)、舒张压(DP)和平均动脉压(MAP)明显升高,但皮下分别注射RES或EST后,SP,DP和MAP明显降低．去势鼠对AngⅡ,NA和Adr升压反应明显减弱,MAP的升高值明显减小．皮下注射RES或EST后,去势鼠对AngⅡ和NA敏感性均有不同程度的恢复,但皮下注射RES后,去势鼠对Adr的反应无明显改善．去势鼠颈动脉窦压力感受性反射的敏感性明显减弱,表现为其升压反射中MAP的升高明显低于假手术大鼠．结果表明:去势鼠静息动脉血压升高,对升压剂AngⅡ,NA,Adr和颈动脉窦压力感受性反射的升压反应明显减弱．皮下分别注射EST结构类似物,即植物雌激素RES后,去势鼠对AngⅡ,NA和颈动脉窦压力感受性反射的升压反应与EST相似均有不同程度的恢复．

The aim of this study was to test the mechanism of cardiovascular protection by investigating the effect of resveratrol and 17β-estradiol on blood pressure and pressor response in ovariectomized rats. We selected female mature Wistar rats weighing 214.07±4.85 g that underwent a bilateral ovariectomy （OVX rats）. Sham-operated animals （SHAM rats） were used as controls. One weeks after surgery, the OVX animals received daily subcutaneous injection of resveratrol （RES, 0.4 mg/kg）, 17β-estradiol （EST, 1 mg/kg） or equivalent volumes of sesame oil for 21 days, the resting arterial blood pressure and pressor responses of angiotensin Ⅱ （Ang Ⅱ, 0.01 mg/kg）, noradrenaline （NA, 0.01 mg/kg） and adrenaline （Adr, 0.016 mg/kg） were detected and the carotid sinus baroreflex was also measured. In OVX rats, systolic pressure （SP）, diastolic pressure （DP） and mean arterial pressure （MAP） significantly increased, but after treatment with RES and EST for 21 days, the blood pressure decreased and almost changed into the level of SHAM rat. Pressor responses of Ang Ⅱ, NA and Adr in SP, DP and MAP were significantly reduced in OVX rats and after treatment with RES and EST, the pressor responses of Ang Ⅱ and NA resumed in varying degrees but for Adr there was no marked change in RES treated OVX rats. The sensitivity of carotid sinus baroreflex significantly decreased in OVX rats, that is to say, changes of SP, DP and MAP were less than SHAM rats. After administration RES and EST, the sensitivity of carotid sinus baroreflex recovered in different degrees. The results indicate that, similar as 17β-estradiol, resveratrol can chronically decrease arterial blood pressure, recover pressor responses of Ang Ⅱ, NA, and the sensitivity of carotid sinus baroreflex in OVX rats and that these effects may be related to its cardiovascular protective effect.