弥漫性大B细胞淋巴瘤中Survivin和bcl-2蛋白表达及相互关系

Expression of surviving protein in DLBCL and its relationship with expression of bcl-2 protein

目的探讨Survivin和bcl-2在弥漫性大B细胞淋巴瘤(diffuselargeBcelllymphoma,DLBCL)免疫类型中的表达及相互关系。方法采用免疫组化MaxVision法检测凋亡抑制基因Survivin和bcl-2在DLBCL免疫类型中和反应性增生的淋巴结中的表达。结果免疫分型60例弥漫性大B细胞淋巴瘤中免疫类型GCB(germinalcenter-B-celllike)25例,non-GCB(nongerminalcenter-B-celllike)35例;免疫类型GCB中survivin的阳性率为72.0%,non-GCB中survivin的阳性率为42.9%,两者之间P<0.05;GCB中bcl-2的阳性率为76.0%,non-GCB中bcl-2的阳性率为31.4%,两者之间P<0.01;survivin阳性弥漫性大B细胞淋巴瘤中bcl-2阳性率为69.7%,survivin阴性弥漫性大B细胞淋巴瘤中bcl-2阳性率为25.9%,在DLBCL中,Survivin的表达与bcl-2蛋白表达密切相关。结论survivin、bcl-2的表达与弥漫性大B细胞淋巴瘤的免疫学类型相关,GCB类型中高表达,non-GCB类型中低表达。在DLBCL中Survivin与bcl-2蛋白表达密切相关,两者协同发挥抗凋亡效应。

Objective To study the expression of survivin protein and its relationship with expression of bcl-2 protein in the immunology types of diffuse large B-cell lymphoma.Methods The expression of surviving in the immunology types of diffuse large B-cell lymphoma, reactive hyperplasia lymphoid node,and the expression of bd-2 in the immunology types of diffuse large B-cell lymphoma were detected by MaxVision immunohistochemical method.Results In 60 specimens of diffuse large B-cell lymphoma patients,immunology type： GCB 25 cases, non-GCB 35 cases;GCB 25 cases, the positive rates of survivin was 72.0% ,non-GCB 35 cases, the positive rates of survivin was 42.9%; GCB 25 cases, the positive rates of bcl-2 was 76.0% ,non-GCB 35 cases, the positive rates of bcl-2 was 31.4%; The positive rates of bcl-2 in survivin positive and negative specimens were 69.7%,25.9% respectively, there being significant difference between the two groups（ r=0.436,P〈0.01）.Conclusion The high expression of survivin and bcl-2 were related to GCB immunology type of diffuse large B-cell lymphoma;The expression of survivin is closely correlated to that of bcl-2 and they may play a synergia role in the course of anti apoptosis.