HPLC法和紫外分光光度法测定葛根淀粉中葛根素含量的研究

Comparative studies on puerarin determination of kudzu roots starch by ultraviolet spectrophotometry and HPLC

葛根淀粉经热水溶解糊化后,用无水乙醇沉降淀粉,然后经大孔树脂分离纯化葛根素后用紫外分光光度法测定葛根素含量,或者用HPLC法直接测定葛根素含量。实验表明对葛根素含量低的样品,紫外分光光度法测定结果较HPLC法偏高,而葛根素含量高的样品,两法测定结果较为接近。因此,对葛根淀粉中葛根素的检测,宜采用HPLC法,而紫外分光光度法用于葛根中葛根素含量的测定,可以替代HPLC法。

Kudzu Roots Starch which was dissolved by hot water, and settied by ethanol absolute,then Macro reticular resins was used to separate and purify puerarin from it, and extract Puerarin by Ultraviolet Speetrophotometry, or determine the content of puerarin by HPLC directly. Experiments showed that the content of Puerarin is low in Kudzu Roots Starch, the Ultraviolet Spectrophotometry＇ s results is more higher than HPLC, hut the higher samples,two results are close. So we may use the HPLC to determinate Puerarin of the Kudzu Roots Starches ,for the content of the Puerarin in wild. Puerarin Lobata,the Ultraviolet Spectrophotometry can substitute the HPLC.