山东籍汉族寻常型天疱疮与HLA-DRB1基因的相关性研究

Association between HLA-DRBl genes and pemphigus vulgaris in Han Shandong Chinese

目的探讨HLA-DRB1、DQB1位点基因在山东籍汉族寻常型天疱疮易感性中的作用。方法用序列特异性引物-聚合酶链反应(PCR-SSP)方法,对61例寻常型天疱疮(pemph igus vu lgaris,PV)患者和57名正常对照者进行了HLA-DRB1、DQB1等位基因的分型,并分析了DRB1、DQB1基因在两组中的分布。结果与正常对照组比较,PV患者组DRB1*14(*1401、*1404*、1405)基因频率明显增高(Pc分别<0.05)。结论HLA-DRB1*14是山东籍汉族PV的易感基因。

Objective To investigate the predisposing role of HLA-DRB1 genes in pemphigus vulgaris （PV）. Methods We used polymerase chain reaction-specific sequence primers method to type HLA-DRB1 ,DQB1 subregion in the patients with PV of Han nationality from Shandong Province and matched control subjects. Results The results demonstrated that DR4, DRB1 ＊ 14 （ ＊ 1401, ＊ 1404, ＊ 1405 ） gene frequencies were significantly higher in PV than those in controls （ Pc 〈 0. 05 ）. Further typing of DR4 positive subjects revealed that the gene frequencies of DRB1 ＊ 0406 were significantly in PV patients compared with controls（ P 〈0. 05）. Conclusion The results suggested that HLA-DRB1 ＊ 14 may be the susceptible haplotypes for PV patients in Chinese Han.