摘要
目的研究转化生长因子β1(TGF-β1)在胆管癌组织中的表达,探讨它与胆管癌生物学行为及预后的关系。方法应用免疫组织化学SP法检测47例胆管癌及癌旁正常胆管组织中TGF-β1的表达,分析其表达与胆管癌临床病理特征的关系,结果进行统计学处理。结果TGF-β1阳性表达主要定位于胆管癌细胞胞浆,偶有胞膜、胞核着色。47例胆管癌组织中TGF-β1阳性表达36例(76.6%),较癌旁正常胆管组织高(P<0.001)。TGF-β1表达与胆管癌临床分期及是否有淋巴结、肝转移有关(P<0.01,0.05,0.05),与组织学分级无关(P>0.05)。结论TGF-β1可能参与了胆管癌的发生、发展。随着胆管癌临床分期的增高而TGF-β1表达增高,其基因缺失可能是胆管癌细胞侵袭性生长转移的重要分子机制,检测TGF-β1表达有助于判断胆管癌的恶性程度及预后。
Objective To study the expression of TGF-β1 in cholangiocarcinoma tissues and its biological behavior. Methods TGF-β1 was detected hy immunohistochemicai methods in 47 paraffin-embedded specimens of cholangiocarcinoma and the control tissues near to the tumor. Results Expression of TGF-β1 was significantly higher in cholangiocarcinoma than that of control tissues near to the tumor( P 〈 0. 001 ). There was significantly relation between TGF-β1 expression and clinical stages, lymph node and liver metastasis( P 〈0. 01,0.05,0.05) ,and no difference in histological grade. Conclusion Smad4 and TGF-β1, TGF-βRⅡ may play an important role in the occurrence and development of cholangiocarcinoma. Loss of Smad4 and TGF-βRⅡ gene may he one of the significant molecular biological mechanism.
出处
《滨州医学院学报》
2006年第6期427-428,431,共3页
Journal of Binzhou Medical University
关键词
胆管肿瘤
转化生长因子Β1
免疫组织化学
bile duct neoplasm,transforming growth faetor-β1,immunohistochemistry