山羊FSH类似物基因构建及其表达

Construction and Expression of Goat Follicle-stimulating Hormone Analogous Gene

以HCGβ亚基羧基末端延长肽CTP基因为连接序列,重组山羊FSHα和β亚基基因,获得单链gFSHβ-CTP-α,克隆入表达载体pVITRO,测序后转染CHO细胞。结果表明,成功完成了单链山羊FSH类似物表达载体的构建,并得到稳定表达的CHO细胞株,其表达量为0.120 mIU/mL,为进一步研究CTP结构与功能的关系,以及长效FSH制剂的研制奠定了分子基础。

The single strand gFSHβ-CTP-α gene was successfully constructed by the combination of the C-terminal peptide （CTP） of carboxyl-terminal region of human chorionic gonadotropin with the goat FSH α-subunit and β-subunit genes. The gene was subcloned into expression vector pVITRO and transfected into Chinese Hamster Ovary （CHO） cells after gene sequencing. The results show that FSH analogous gene expression vector was successfully constructed , the CHO cells expressed gFSHβ-CTP-α were acquired and the quantity of expression is 0. 120 mIU/mL. These results would lay the molecular foundation for studying the relation between structure and function of CTP and the production of long-acting FSH.