牛磺酸对大鼠睾丸间质细胞分泌睾酮的影响及作用机理初探

Effects of Taurine on Testosterone Secretion and Its Mechanism

将雄性Wistar大鼠（220～250g）的睾丸间质细胞混悬液用不连续梯度（5％、30％、58％、70％）Percoll分离液分离，在细胞培养液中分别加入牛磺酸及促性腺激素（HCG）、雄烯二酮、孕酮、cAMP进行培养，用放射免疫分析方法测定作用24h后培养液中睾酮含量。结果表明，牛磺酸可使睾丸间质细胞分泌睾酮的能力显著增加；能增加HCG和cAMP诱导的睾酮分泌；能促进雄烯二酮和孕酮向睾酮的转化。牛磺酸能增加睾丸间质细胞睾酮的分泌，并可能在类固醇合成的多个环节中都起到了一定的促进作用。

Leydig cells were isolated and purified by a discontinuous Percoll gradient （5%, 30%, 58%, 70%）. Purified cells were dispensed into 24-well plate and maintained for 24h in a COz incubator. Leydig cells were treated with taurine independently or coadministered with human chorionic gonadotropin （HCG） ,androstenedione,progesterone and cyclic adenosine monophosphate（cAMP）. Testosterone levels were measured by radioimmunoassay. The results showed that taurine can affect testosterone secretion significantly in a biphasic pattern. Taurine at the concentration （0. 1- 100 μg/mL） increased testosterone secretion , whereas the higher dose （400μg/mL）inhibited testosterone secretion. Taurine can stimulate the convertion from androstenedione and progesterone to testosterone, which can also stimulate the testosterone secretion induced by HCG and cAMP. It is concluded that taurine can stimulate testosterone secretion ,which affect several aspects of testosterone biosynthesis.