晕船及适应过程中大鼠血浆及脑氨基酸含量变化

CHANGES OF FREE AMINO ACIDS IN PLASMA AND BRAIN OF RATS DURING SIMULATED SEA SICKNESS AND POST-ADAPTATION

目的:通过观察模拟晕船及适应后大鼠血浆及脑内游离氨基酸含量变化,为进一步研究防治晕船的营养干预措施提供实验依据。方法:采用Crampton模型模拟晕船刺激,以异嗜高岭土行为作为判定大鼠晕船及晕船适应的指标,运用高效液相色谱法测定晕船及晕船适应过程中大鼠血浆及脑内游离氨基酸含量变化。结果:模拟晕船刺激1d后,血浆胱氨酸和异亮氨酸含量显著升高,Gly、Pro含量显著下降,BCAA/AAA的比值升高;脑内Ala、Cys+Met、Tyr、His以及总氨基酸含量显著下降;模拟晕船刺激21d后血浆及脑内游离氨基酸无明显变化,脑内Glu/GABA显著升高。晕船耐受与晕船易感大鼠血浆及脑内游离氨基酸含量、血浆BCAA/AAA比值及脑内Glu/GABA比值均无显著差异。结论:晕船刺激使实验大鼠血浆和脑内氨基酸含量发生明显改变,其中有些与神经递质合成有关的氨基酸改变较显著。

Objective： To observe the changes of free amino acids （FAAs） in plasma and brain of rats during simulated sea sickness and post-adaptation. Method： Pica or kaolin consumption was used as an indicator to judge the development of sea sickness and adaptation when SD rats were stimulated by Crampton sea sickness simulator. FAAs concentrations in plasma and brain of rats were determined by high performance liquid chromatography （HPLC） after simulated sea sickness and post-adaptation. Results： After simulated sea sickness stimulation for 1 d, the levels of Cys and Ile were increased, and Gly and Pro decreased significantly in plasma. The increase of BCAA/AAA ratio was also found. In brain, the contents of Ala, Cys＋Met, Tyr, His, and total amino acids were remarkably decreased. After simulated sea sickness stimulation for 21 d, no changes of FAAs were observed in plasma and brain but the ratio of Glu/GABA was increased in brain. There were no differences of FAAs, Glu/GABA ratio and BCAA/AAA ratio in plasma and brain of tolerant and susceptible sea sickness rats. Conclusion： The levels of amino acids, especially those related to neurotransmitter synthesis, in plasma and brain were changed significantly during sea sickness.