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成人骨髓间充质干细胞培养上清对人脐血间充质干细胞体外培养及扩增的支持作用 被引量:4

Sustaining effect of cultured adult marrow mesenchymal stem cells supernatant on culture and proliferation of mesenchymal stem cells from human umbilical cord blood in vitro
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摘要 目的:研究人骨髓间充质干细胞培养上清对人脐带血(HUCB)中所含有间充质干细胞(MSCs)的影响。方法:取脐带血,肝素抗凝,Percoll淋巴细胞分离液分离出单个核细胞,低糖DMEM培养获得贴壁细胞层。与人骨髓间充质干细胞培养上清共孵育。流式细胞仪检测表面抗原。结果:脐带血的单个核细胞与人骨髓间充质干细胞培养上清共孵育经体外培养贴壁后出现形似纤维状细胞形态并表达与MSCs相关的抗原(CD13,CD44,CD71,CD166),但不表达造血细胞抗原(CD34,CD45),这与源于骨髓的MSCs一致。结论:人脐血间充质干细胞与骨髓间充质干细胞培养上清共孵育,对脐血间充质干细胞体外分离培养及扩增有支持作用。 Objective:To study the effect of cultured human marrow mesenchymal stem cells supematant on human umbilical cord blood mesenchymal stem cells culture. Method: Umbilical cord blood was acquired, heparins was used for anti - coagulation, monocytes were isolated by Percoll solution, and low - glucose DMEM culture was used to acquire adhere cells layer. The monocytes were co -incubated with supernatant from human marrow mesenchymal stem cells culture, and flow cytometer was used to detect surface antigen. Result: After the monocytes of human umbilical cord blood were co - incubated with supernatant of human marrow mesenchymal stem cells, the shape of cells adhered to layer mimicked that of fibrocytes. These cells expressed MSCs - related antigens (CD13, CD44, CD71, CD166), but did not express hematopoietic cell antigens (CD34, CIM5), which was similar with that of marrow mesenchymal stem cells. Conclusion: Human umbilical cord blood mesenchymal stem cells co - incubated with the supernatant of human marrow mesenchymal stem cells can promote the culture and proliferation of umbilical cord blood mesenchymal stem cells in vitro.
出处 《西北国防医学杂志》 CAS 2006年第6期447-449,共3页 Medical Journal of National Defending Forces in Northwest China
基金 兰州军区医药卫生科研基金资助项目(LXH-2005013)
关键词 人脐带血 间充质干细胞 体外培养 Human umbilical cord blood Mesenchymal stem cells Culture in vitro
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参考文献6

  • 1Erices A,Conget P,Mingue J.Mesenchymal progenitor cells in human umbilical cord blood[J].Bri J Haematology,2000,109:235-242.
  • 2Liechty KE,Mackenzie TC,Shaaban AF,et al.Human mesenchymal stem cells engraft and demonstrate site specific differentiation after in utero transplantation in sheep[J].Nat Med,2000,6(11):1282 -1286.
  • 3侯玲玲,曹华,魏国荣,白慈贤,张涌,吴祖泽,裴雪涛.人脐血间充质干细胞体外扩增和向神经元样细胞定向诱导分化的研究[J].中华血液学杂志,2002,23(8):415-419. 被引量:75
  • 4Wexler SA,Donaldson C,Denning Kenda P,et al.Adult bone marrow is a rich source of human mesenchymal stem cells but umbilical cord and mobilized adult blood are not[J].Br J Haematol,2003,121:368-374.
  • 5Majumdar MK,Thiede MA,Haynesworth SE,et al.Human marrow derived mesenchymal stem cells (MSCs) express hematopoietic cytokines and support long term hematopoiesis when differentiated toward stromal and osteogenic lineages[J].J Hematother Stem Cell Res,2000,9:841-848.
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二级参考文献8

  • 1Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol,2000, 28: 875-884.
  • 2Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol, 2000, 109: 235-242.
  • 3Woodbury D, Schwarz EJ, Prockop DJ, et al. Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res, 2000, 61: 364-370.
  • 4Pereira RF, O'Hara MD, Laptev AV, et al. Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta. Proc Natl Acad Sci U S A, 1998,95:1142-1147.
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  • 6Huss R, Lange C, Weissinger EM, et al. Evidence of peripheral blood-derived, plastic-adherent CD34 (-/low) hematopoietic stem cell clones with mesenchymal stem cell characteristics. Stem Cell, 2000, 18: 252-260.
  • 7Majumdar MK, Thiede MA, Mosca JD, et al. Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol, 1998,176:57-66.
  • 8Ishii K, Katayama M, Hori K, et al. Effects of 2-mercaptoethanol on survival and differentiation of fetal mouse brain neurons cultured in vitro. Neuroscience Letters, 1993,163:159-162.

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