成人骨髓间充质干细胞培养上清对人脐血间充质干细胞体外培养及扩增的支持作用

Sustaining effect of cultured adult marrow mesenchymal stem cells supernatant on culture and proliferation of mesenchymal stem cells from human umbilical cord blood in vitro

目的:研究人骨髓间充质干细胞培养上清对人脐带血(HUCB)中所含有间充质干细胞(MSCs)的影响。方法:取脐带血,肝素抗凝,Percoll淋巴细胞分离液分离出单个核细胞,低糖DMEM培养获得贴壁细胞层。与人骨髓间充质干细胞培养上清共孵育。流式细胞仪检测表面抗原。结果:脐带血的单个核细胞与人骨髓间充质干细胞培养上清共孵育经体外培养贴壁后出现形似纤维状细胞形态并表达与MSCs相关的抗原(CD13,CD44,CD71,CD166),但不表达造血细胞抗原(CD34,CD45),这与源于骨髓的MSCs一致。结论:人脐血间充质干细胞与骨髓间充质干细胞培养上清共孵育,对脐血间充质干细胞体外分离培养及扩增有支持作用。

Objective：To study the effect of cultured human marrow mesenchymal stem cells supematant on human umbilical cord blood mesenchymal stem cells culture. Method： Umbilical cord blood was acquired, heparins was used for anti - coagulation, monocytes were isolated by Percoll solution, and low - glucose DMEM culture was used to acquire adhere cells layer. The monocytes were co -incubated with supernatant from human marrow mesenchymal stem cells culture, and flow cytometer was used to detect surface antigen. Result： After the monocytes of human umbilical cord blood were co - incubated with supernatant of human marrow mesenchymal stem cells, the shape of cells adhered to layer mimicked that of fibrocytes. These cells expressed MSCs - related antigens （CD13, CD44, CD71, CD166）, but did not express hematopoietic cell antigens （CD34, CIM5）, which was similar with that of marrow mesenchymal stem cells. Conclusion： Human umbilical cord blood mesenchymal stem cells co - incubated with the supernatant of human marrow mesenchymal stem cells can promote the culture and proliferation of umbilical cord blood mesenchymal stem cells in vitro.