摘要
采用荧光素染色法，以ＡＣＡＳ５７０型粘附细胞仪研究ＡＴＰ敏感性钾通道特异性激动剂ｃｒｏｍａｋａｌｉｍ对正常心肌细胞内Ｃａ２＋的作用及对缺氧再给氧时细胞内Ｃａ２＋的影响。结果显示：ｃｒｏ－ｍａｋａｌｉｍ降低正常心肌细胞内Ｃａ２＋的浓度；同时明显减轻缺氧再给氧时细胞内Ｃａ２＋超载现象。二者均呈良好的量效关系。提示：ｃｒｏｍａｋａｌｉｍ可能有减轻缺氧再给氧损伤的作用。
Byusingfluorescencestainingmethod,theefectofcromakalimonintracelularfrecalcium(Ca2+)levelinculturedneonatalratmyocardialcelsandinanoxia-reoxygenationmyocardialcelswerestudiedwithadherentcelanalysisandsortingcytometer570(ACAS570).Theresultsdemonstratedthat(1)ThelevelofintracelularCa2+decreasedimmediatelyaftertheadditionofcro-makalim;(2)CromakalimsignificantlyreducedthelevelofintracelularCa2+duetoanoxia-reoxy-genation.Aloftheseweredose-dependent.Theseshowedthatcromakalimcouldagainstcalciumoverloadduringanoxia-reoxygenation.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
1996年第4期302-304,共3页
Chinese Journal of Cardiology
关键词
钙
缺氧
心肌细胞
calciumanoxia-reoxygenationinjurycromakalim
