鼠牙髓组织细胞培养的生长特性及形态特征研究

The Proliferation and Morphologic Characteristics of in Cultured Rat Dental Pulp Cells

应用组织块培养法，对大白鼠牙髓组织细胞进行了原代和传代培养，同时对培养细胞来源、生长特性进行了初步观察和鉴定。结果证实，培养的鼠牙髓细胞为来自中胚层的成纤维细胞，该细胞的细胞器发达，４～５代后的细胞生长稳定，增殖速度快，提示培养的鼠牙髓细胞适合体外实验并以选择４～５代后的细胞进行实验为宜。

Maxillary incisor pulp cells from male wistar rats were cultured and the origin of cultured pulp cells was identified. Results confirmed that cultured pulp cells from rats were fibroblasts. After 4～5 nd subculture, the proliferation of cells was cultured elerated and the growth was steady, These suggested that pulp cells from rats were suitable to experiments in vitro and the selection of the 4～5 nd succesive cells might be more satisfactory.