DNA分子标记技术在花生品种鉴定上的应用研究

Utilization of DNA Molecular Marker Technology in the Discrimination of Peanut Cultivars

利用10个中国花生品种,评估了AFL,SRAP,SSR和STS技术在区分相近来源花生品种上的应用效果。结果表明,AFLP和SRAP标记虽然可以揭示这些花生品种的遗传多样性,但在本研究采用的引物对条件下,单独使用任何一对引物不足以区分全部品种,提示我们需要考虑多对引物的配合,或者需要尝试更多的限制酶或引物对;从103对SSR或STS引物中筛选出9对引物,即Lec_1,Ah4_26,Ah4_4,SsS14,SHPAL_1,PG_71,PG_43,PM_36,PG_22,对所采用的10个花生品种的区分率均为100%。鉴于花生栽培种形态学、细胞学和生化标记贫乏,这9对高辨别力的SSR和STS引物对花生遗传研究和品种鉴定工作具有重要价值。

Ten peanut cuhivars from China were utilized in an attempt to evaluate the discrimination power of different molecular marker systems, including AFLP, SRAP, SSR and STS. AFLP and SRAP technology failed to identify individual peanut cuhivar using a single primer pair, suggesting that more primer pairs and/or restriction enzyme combina- tions are needed. Nine primer pairs, viz, Lec-1, Ah4-26, Ah4-4, SsS14, SHPAL-1, PG-71, PG-43, PM-36, PG-22, resulting from screening of 103 SSR/STS primer pairs proved to have the discrimination power of 100%. Owing to the limited number of morphological, cytogenetic and biochemical markers in cultivated peanut, the 9 informative primer pairs are of significance in peanut genetic and variety identification studies.