甜樱桃体细胞胚胎发生及植株再生的研究

Somatic Embryogenesis of Sweet Cherry and Plantlet Regeneration

以甜樱桃红灯为材料,幼果用0.1%HgCl2进行消毒,在无菌条件下取出未成熟胚子叶进行诱导,在胚成苗后进行继代和生根培养。培养结果表明,以MS为基本培养基,在2,4-D为2.0 mg/L时培养基上胚性愈伤组织诱导率较高,为53.7%;愈伤组织经过数次继代后转移至MS附加6-BA 1.0 mg/L和NAA 0.1 mg/L培养基上可诱导体细胞胚的形成,诱导率可达81.0%.体细胞胚在合适的培养基上可继续生长和增殖。

Cultivars sweet cherry Hongdeng was used as the materials. An immature pterodia was taken from young fruit disinfected with 0.1% HgCl2. Shoot muhiplication culture and rooting culture were conducted after emergence of seedlings. The resuh indicates that the embryogenic callus（EC） frequency was 53.1. % at 2 mg/L of 2,4-D added in MS medium, After subcultured for several times, somatic embryogenesis at high frequency （81.0%） was obtained on MS medium supplemented with 6-BA 1.0 mg/L ＋ NAA 0.1 mg/L. Somatic embryos can keep on growing and proliferating on appropriate medium.