胆盐水解酶合成条件的优化

Studies on the synthesizeation optimization of bile salt hydrolase

以MRS,MRS肉汤,改良MRS为基础培养基,考察了培养基对菌株产胆盐水解酶活力的影响,确定了MRS肉汤为基础培养。对KTx在MRS肉汤中最适碳源、氮源、培养温度、培养基起始pH值、刺激因子作了单因素试验,得出最适碳源为葡萄糖,最适氮源为大豆蛋白胨,最适培养温度37℃,最适培养基起始pH值为5.5～6.0,最适刺激因子吐温80。在此基础上选择葡萄糖、大豆蛋白胨、培养温度、接种量进行L16(44)四因素四水平正交试验。结果表明,对胆盐水解酶活力影响因子水平:葡萄糖>大豆蛋白胨>培养温度>接种量;最佳产胆盐水解酶条件:葡萄糖4%,大豆蛋白胨2%,接种量2%(均为质量分数),培养温度为37℃。优化后菌株的产酶活力可提高到优化前的13.4倍。

To optimize the activity of bile salt hydrolase,research was done on the respect of incubation condition and the media components. And the optimum temperature was 37 ℃, the optimum broth initial pH was 5.5～6.0. The optimum media component was： glucose 40 g, soybean peptone 20 g, meat extract 10 g, Tween80 1 mL, K2HPO4 2 g, Na2Ac 5 g, Tri-Ammonium citrate 2 g, MgSO4 0.58 g MnSO4 0.25 g, H2O 1 000 mL. Production of bile salt hydrolase under the optimum broth was 13.4 times than MRS media.