红叶石楠组织培养工厂化扩繁技术研究

Study on Mass Reproduction of Photinia Fraseri through Tissue Culture

以红叶石楠的侧芽和顶芽为材料进行组织培养试验。结果表明：外植体用0．1%HgCl_2消毒10～12 min,诱导培养基以MS+0．05 mg．L^1 NAA+2．0 mg·L^1 6-BA为佳,芽增殖培养基以MS+1．0 mg·L^1BA+0．1 mg’L^1 6-BA为佳,壮苗培养基以MS+0．5 mg·L^1 NAA+0．5 mg·L^1 6-BA为佳,生根培养基以1/2MS+1．0 mg·L^1 IBA为佳,将生根苗移入温室的穴盘中,基质以蛭石：珍珠岩：泥炭土=5：3：2较好,控制好室内的温度、光照和湿度,成活率可达90%以上。

The paper reports a tissue culture of Photinia Fraseri with its side-bud or top-bud. The results showed that the better sterilization is putting in 0. 1% HgCl2 solution for 10-15 mint the better inducement medium is MS＋0. 05 mg · L^-1 NAA＋2. 0 mg· L^-1 6-BA the better buds multiplication medium is MS＋0. 0 mg · L^-1BA＋0. 1 mg · L^-1 6-BA the better medium for strong seedling is MS＋0. 5 mg · L^-1 NAA＋0. 5 mg · L^-1 6-BA; the better medium for striking roosts is 1/2MS ＋ 1.0 mg · L^-1 IBA the better base soil is composed of vermiculite ： pearlite ： peat=5 ： 3 ： 2 after struck roots seedlings being transplanted to hole plates in greenhouse. The survival rate can be over 90% under well-controlled temperature and humidity.