摘要
以红叶石楠的侧芽和顶芽为材料进行组织培养试验。结果表明:外植体用0.1%HgCl_2消毒10~12 min,诱导培养基以MS+0.05 mg.L^1 NAA+2.0 mg·L^1 6-BA为佳,芽增殖培养基以MS+1.0 mg·L^1BA+0.1 mg’L^1 6-BA为佳,壮苗培养基以MS+0.5 mg·L^1 NAA+0.5 mg·L^1 6-BA为佳,生根培养基以1/2MS+1.0 mg·L^1 IBA为佳,将生根苗移入温室的穴盘中,基质以蛭石:珍珠岩:泥炭土=5:3:2较好,控制好室内的温度、光照和湿度,成活率可达90%以上。
The paper reports a tissue culture of Photinia Fraseri with its side-bud or top-bud. The results showed that the better sterilization is putting in 0. 1% HgCl2 solution for 10-15 mint the better inducement medium is MS+0. 05 mg · L^-1 NAA+2. 0 mg· L^-1 6-BA the better buds multiplication medium is MS+0. 0 mg · L^-1BA+0. 1 mg · L^-1 6-BA the better medium for strong seedling is MS+0. 5 mg · L^-1 NAA+0. 5 mg · L^-1 6-BA; the better medium for striking roosts is 1/2MS + 1.0 mg · L^-1 IBA the better base soil is composed of vermiculite : pearlite : peat=5 : 3 : 2 after struck roots seedlings being transplanted to hole plates in greenhouse. The survival rate can be over 90% under well-controlled temperature and humidity.
出处
《山西农业大学学报(自然科学版)》
CAS
2006年第4期332-334,共3页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
镇江市科技成果示范推广项目(NY2004032)
关键词
红叶石楠
外植体
组织培养
Photinia Fraseri Explant Tissue culture