HPLC法测定黄精赞育胶囊中四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量

Determination of Tetrahydroxystilbene-2-O-β-D-Glucoside in Huangjing Zanyu Capsule by HPLC

目的建立黄精赞育胶囊中(制何首乌、黄精等)四羟基二苯乙烯-2-O--βD-葡萄糖苷的含量测定方法。方法采用HPLC法,Lichrospher 5-C18柱(250 mm×4.6 mm,5μm);流动相为乙腈-水(22∶78);检测波长320 nm。结果四羟基二苯乙烯-2-O--βD-葡萄糖苷的线性范围在8.09μg^1.62μg,r=0.9999,平均回收率为98.8%,RSD为1.74%。结论该法简便快捷,准确实用,可用于黄精赞育胶囊的质量控制。

OBJECTIVE To establish a method for the determination of tetrahydroxystilbene-2-O-β-D-gluccside in Huangjing Zanyu capsule. ME＂THODS ~ assay was performed in Liehrospher 5-C18 column（250 mm × 4.6 mm,5 μm） with a mobile phase conisted of CH3CN-H2O （22：78）, and the detector wavelength was set at 320 nm. RESULTS The linear range of tetrahydroxystilbene-2-O-β-D-gluccside was 8.09 μg- 1.62 μg and the relation coefficient was 0.9999. The average recovery rate was 98.8%, RSD was 1.74%. CONCLUSION The method was simple, accurate and reliable in quality control of Huangjing Zanyu capsule.