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中国明对虾(Penaeus chinensis kishinouye)Sox基因PCR-SSCP分析条件优化的研究 被引量：1

A Study on Optimization of the Conditions Affecting SSCP Analysis of Sox Gene in Penaeus chinensis Kishinouye
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摘要 聚合酶链反应—单链构象多态性(PCR-SSCP)是一种快速、简便、较灵敏的检测基因变异的方法,但其影响因素很多,本试验从多个方面对中国对虾SOX基因SSCP方法的影响因素进行分析,结果发现应用12%,交联度49:1的聚丙烯酰胺凝胶常温下低压电泳效果最佳,条带清晰,容易识别,可应用于其他SSCP分析方法的参考。
作者 朱琴 汪桂玲
机构地区 上海水产大学生命科学与技术学院
出处 《现代渔业信息》 2006年第12期7-9,共3页 Journal of Modern Fisheries Information
基金 上海市水产养殖重点学科建设项目(Y1101) 上海市教委青年科研基金(03NQ10) 校级水产养殖重点学科基金(03-40)资助
关键词 中国对虾 SSCP 聚丙烯酰胺凝胶
分类号 S917.4 [农业科学—水产科学]
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参考文献12

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二级参考文献34

共引文献27

同被引文献35

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现代渔业信息
2006年 第12期

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