吗啡预处理对缺血/再灌注后大鼠小脑神经元的保护作用

The protection effect of morphine preconditions to cerebellar nerons in rats from ischemia reperfusion

Objective To determined whether morphine preconditioning induces ischemic tolerance in neron.Methods We used rat cerebellar slices and simulated ischemia by oxygen-glucose deprivation(OGD),Slices were kept in artifical cerebrospinal fluid(aCSF) containing various concentrations of morphine(0.1 μmol/L^1μmol/L),Then they were transferred to aCSF without glucose and oxygen.After 5 h,slices were fixed for morphology examination.To determine the percentage of undamaged Purkinje cell(survival rate).Purkinje cells were recognized based on the morphology and the location.At least 50 Purkinje cells were counted from each of the sections and cells in total three sections were counted for each slice(>150 Purkinje cells).Results The survival rate of Purkinje cells was significantly higher in group preconditioned with morphine(survival rates were 56%) than that of the oxygen-glucose deprivation(survival rates were39%).χ~2=293,P<0.01.The protective effects of morphine preconditioning were concentration dependent and were maximal at 0.3 μmol/L.Conclusion Morphine pretreatment can induces ischemic tolerance in neurons,but also the characteristic were the roof effects.

Objective To determined whether morphine preconditioning induces ischemic tolerance in neron. Methods We used rat cerehellar slices and simulated ischemia by oxygen-glucose deprivation （OGD）, Slices were kept in artifical cerehrospinal fluid （aCSF） containing various concentrations of morphine （0.1 μmol/L～1 μmol/L）, Then they were transferred to aCSF without glucose and oxygen. After 5 h, slices were fixed for morphology examination. To determine the percentage of undamaged Purkinje cell （survival rate）. Purkinje cells were recognized based on the morphology and the location. At least 50 Purkinje cells were counted from each of the sections and cells in total three sections were counted for each slice （〉 150 Purkinje cells）. Results The survival rate of Purkinje cells was significantly higher in group preconditioned with morphine （survival rates were 56%） than that of the oxygen-glucose deprivation （survival rates were 39%）. χ^2 = 293, P 〈 0.01. The protective effects of morphine preconditioning were concentration dependent and were maximal at 0.3 μmol/L. Conclusion Morphine pretreatment can induces ischemic tolerance in neurons, but also the characteristic were the roof effects.