Survivin在大肠癌中的表达及其与肿瘤血管形成的关系

The expression of survivin in colorectal carcinoma and relation with neovascularization

目的检测大肠癌中凋亡抑制基因Survivin的表达,研究其与大肠癌临床特征和碱性成纤维细胞生长因子(bFGF)表达的相关性,探讨Surviyin在大肠癌发生、发展及转移中的作用及机制。方法收集60例大肠癌标本及其病例资料,应用免疫组织化学方法检测Survivin,bFGF在大肠癌标本中的表述情况。结果(1)大肠癌中Survivin和bFGF的阳性表达率分别为78.33%、65.00%,明显高于癌旁正常大肠组织(P<0.05);(2)Survivin的表达与大肠癌的Dukes分期及淋巴结转移明显相关(P<0.05),而与大肠癌的病理分级无关(P>0.05);(3)bFGF的表达与大肠癌的Ducks分期、淋巴结转移明显相关(P<0.05),而与大肠癌的病理分级无关(P>0.05);(4)Survivin与bFGF在大肠癌中的表达呈正相关(P<0.05,r=0.364),二者的联合表达与大肠癌的微血管密度(IMVD)密切相关(P<0.05)。结论Survivin和bFGF可能在大肠癌的发生发展中起关键作用,二者与大肠癌的临床病理分期密切相关,二者联合检测对大肠癌的诊断有一定价值。

Objective To investigate the expression of survivin and bFGF in human coloreetal carcinoma tissue and evaluate their value as a tumor marker and clinical prognostic indicator. Methods The protein expression of survivin and bFGF were examined in 60 colorectal carcinoma by immunohistochemical method （SP technique）. Remits （ 1 ） In eolorectal carcinoma the expression rote of survivin and bFGF index in protein levelwas 78.33% and 65.00% respectively, which were higher than that in normal eolorectal tissues （ P 〈 0.05）. （2）The expression of survivin gene significantly correlate with the Dukes stage, lymph node status （ P 〈 0. 05 ）, but have no correlation with pathological grades （ P 〉 0.05）. （3） The expression of bFGF gene significantly correlate with the Dukes stages, lymph node status （ P 〈 0.05）, but have no correlation with pathological grades （ P 〉 0,05）. （4）There was a significantly positive correlation between survivin and bFGF （ P 〈 0.05, r = 0.364）, the expression of survivin and bFGF significantly correlate with IMVD （ P 〈 0.05）. Conclusion The expression of survivin and bFGF were significantly related to the occurrence and progression of colorectal carcinoma, that may serve as a definte diagnstic marker for colorectal carcinoma.