反相离子对色谱-脉冲安培电化学法测定硫酸庆大霉素中各组分含量

Analysis of Gentamicin Sulfate by Liquid Chromatography with Pulsed Amperometric Detection

建立了一种新的用反相离子对液相色谱(LC)分离,以金电极为工作电极的脉冲安培电化学法(PAD)直接检测硫酸庆大霉素中各组分含量的分析方法。流动相为0.033 mol/L草酸、0.012 mol/L七氟丁酸、210 mL/L乙睛,用稀NaOH调节pH至3.4。与报道的其它方法相比,该方法能使庆大霉素各有效组分C1、C1 a、C2、C2 a很好分离,整个分析过程<30 m in。考察了各色谱参数对分离测定的影响。实验证明,本方法不需要衍生化,可直接检测硫酸庆大霉素。

A reversed phase ion pair liquid chromatography （LC） method with pulsed amperometric detection on a gold electrode after post added NaOH is described. The mobile phase consisted of an aqueous solution containing 0. 033 mol/L oxalic acid, 0.012 mol/L heptafluorobutyric acid and 210 mol/L acetonitrile with pH 3.40 adjusting by dilute NaOH solution. The total analysis time was less than 30 min, and the method allows a better separation of gentamicin C1, C1a, C2, and C2a. The effects of the different chromatographic parameters on the separation were also investigated.