链霉素聚氰基丙烯酸正丁酯纳米粒的体外跨膜转运研究

In vitro membrane transport of streptomycin polybutylcyano-acrylate nanoparticles

目的:探讨纳米化对链霉素的胞内渗透率和胞内浓度的影响。方法:以氰基丙烯酸正丁酯为聚合材料,通过乳化聚合法制备链霉素纳米粒,以人外周血单核细胞为体外细胞模型,采用高效液相色谱-质谱法检测链霉素的胞内浓度,对链霉素纳米粒和链霉素的跨膜转运情况进行对比考察。结果:链霉素纳米粒组的链霉素透过率和链霉素胞内浓度在各时间点均明显高于链霉素组,至16 h时,链霉素纳米粒组链霉素的胞内浓度为链霉素组的两倍,两组之间具有显著性差异(P<0.05)。结论:纳米化能增强链霉素的胞内渗透能力,提高链霉素的胞内浓度,使进入细胞内的链霉素浓度增加。

Aim：To investigate the penetrability of the prepared streptomycin polybutyleyano-acrylate nanoparticles in the cultured cells. Methods： Streptomycin polybutylcyano-acrylate nanoparticles were prepared by the emulsification polymerization method used polybutylcyanoacrylate as carrier. In the cultured cells of peripheral blood monocytes, LCMS was used to assay the intracellular concentrations of streptomycin thereby investigating the in vitro penetrability of streptomycin before and after nanoparticlized. Results： It was found that, at the preplanned intervals, all of determined cellular streptomycin concentration in the treatment with the nanoparticles was higher than that of streptomycin alone. At 16th hour, intracellular streptomycin concentration in the treatment of the nanoparticles was twice than that of streptomycin alone （ P 〈 0, 05 ）, Conclusion： The results indicated that penetrability of streptomycin across the cellular membrane has been facilitated by nanoparticlized.