山葡萄“双优”品种组培苗脱毒方法的研究

Virus-eliminated methods for the seedlings of "Shuang You" in Vitis amurensis Rupr

以山葡萄“双优”品种组培苗为材料，采用热处理与茎尖培养相结合的方法，比较不同处理时间、不同茎尖长度对组培苗脱毒茎尖成活率的影响，同时筛选茎尖脱毒培养的最适合培养基．结果表明：在葡萄茎尖脱毒培养中，最适培养基为：MS＋6-BA2mg／L＋NAA0．05mg／L＋蔗糖30girl＋琼脂9g/L．采用热处理20～30d的茎尖成活率较单纯茎尖培养（0．2mm）成活率也有增加，且热处理时间20～30d，茎尖大小在0．4mm左右时的组培苗长势好，死亡率几乎为零，茎尖成活率比较理想．

The tube seedlings of ＂Shuang You＂in Vitis amurensis Rupr were selected as the method of heat treatment combined with tip culture to study the effects of different heat treatments and different tip lengths on the survival rate of shoot tips and the suitable medium for virus-eliminated tip culture was obtained. The results showed that the suitable medium for microapical culture was MS ＋ 6-BA 2 mg/L ＋ NAA 0.05 mg/L ＋ sucrose 30 g/L ＋ agar 9 g/L. The tip survival rate under heat treatment for 20- 30 days was higher than single tip culture （0.2 mm）. Moreover, when tip length was 0.4 mm and the time for heat treatment was 20 30 days, tube seedlings grew well, and the death rate was almost zero and tip survival rate was good.