玻片凝集和微量凝集反应板试验在肥达-外斐试验中的应用研究

Applied research on plate-glass coagulating and micro-coagulating reaction plate test in Widal and Weil-Felix test

目的探讨用玻片凝集和微量凝集反应板试验在肥达-外斐试验中的使用,诊断7种发热病菌感染(如沙门菌病和某些立克次体感染病等)中的应用价值,以及在急诊和部队野外卫勤保障中的前瞻性研究。方法对临床诊断为伤寒的78例患者和发热的42例患者先进行急诊筛选试验(即玻片凝集试验),然后用微量凝集反应板试验进行确证试验。结果这种急诊筛选试验简便快速、有初步定性筛选作用;微量凝集反应板试验特异性强,交叉反应率低,具有定性和半定量双重功效,能准确检测7种常见发热病菌抗原的抗体。结论玻片凝集和微量凝集反应板试验以其迅速、准确、操作性强的特点,将在发热病菌感染的急诊诊断和野外诊疗中发挥作用。

Objective To discuss the applied value of plate-glass coagulating and micro-coagulating reaction plate test for seven kinds of febrile infection. Methods Emergency screening test was performed in 78 eases of typhoid diagnosed in clinic and 42 eases of febrile infection,then the definitive test was performed with micro-coagulating reaction plate test. Results This emergency screening test is simple and rapid, and has preliminary qualitative effect. Micro-coagulating reaction plate test has high sensitivity, specificity and double functions of qulitation and semi-quantitatlon. The rate of cross-reaction is lower. Conclusion This method has the characteristics of rapidity, precision and easy manipulation for the diagnosis of febrile infection in emergency and field.