广西象州温泉嗜热菌的分离与鉴定

Separation and Identification of Thermophilic Bacterium Strain from Xiangzhou Hot Springs of Guangxi

采集广西象州温泉水样，用常规的稀释涂布法分离和纯化嗜热菌，并选择其中1株编号为XZ2K2的菌株进行形态观察、生理生化鉴定和16SrDNA序列分析。结果从温泉水中分离得到9个菌株，XZ2K2菌株的革兰氏染色阳性，无芽孢，细胞呈杆状，最适生长温度为70-75℃，最适的生长pH值为7．0～7．5．初步鉴定该菌株为布氏栖热菌（Thermus brockianus）。

In order to separate and identify the thermophilic le.cteria in hot springs of Xiangzhou, Guangxi, the general method of dilution spread was used. One strain was selected and labelled as XZ2K2. Morphological observation, physiology-biochemistry tests and 16SrDNA sequence analysis have been conducted. Nine strains were separated from the hot springs. XZ2K2 is bacillus,grampositive, non-sporulation. Its optimum temperature for growth was between 70℃ and 75℃, optimum pH for growth was between 7.0 and 7.5. XZ2K2 was identified as Thermus brockianus.