新抑癌基因ARHI在子宫内膜异位症中的表达及其意义

Expression and significance of a noval tumor suppressor gene ARHI in endometriosis.

目的:研究子宫内膜异位症组织中抑癌基因ARHI蛋白的表达。方法:选择抗ARHI单克隆抗体,采用W estern blot免疫印迹法,对10例子宫内膜异位症的异位囊肿组织及其在位内膜组织进行蛋白检测,并以10例正常女性的在位内膜组织作对照。用Array 3000扫描仪扫描X线片,观察W estern blot的结果,用GDS8000型凝胶成像分析系统分析图片的辉度值和比值。结果:(1)ARHI在内异症异位内膜的表达高于在位内膜(P<0.001)及正常内膜(P<0.001);(2)内异症的在位内膜组与正常对照组比较,ARHI的表达无明显差异(P>0.05)。结论:抑癌基因ARHI在子宫内膜组织中普遍表达,且在异位内膜中表达上调,提示ARHI基因可能与子宫内膜异位症的发病及进展相关。

Objective：To investigate ARHI expression in endometriosis, and discuss the invasion mechanism of endometriosis. Methods：Ten specimens of ectopic and eutopic endometrium with endometriosis, and ten control members were investingated by using western blot. Array 3000 scanning apparatus scaned the X-ray films. The gray scale and the ratio were detected and analysed gy GDS8000 gal image analysis system. Results： （ 1 ） The expression of ARHI protein in ectopic endometrium group was extremely higher than that in eutopic group （P 〈 0.001 ） and that in normal endomerium group （P 〈0.001 ） ;（2） The expression of ARHI protein in eutopic endometrium group was not different with that in eutopic group （ P 〉 0.05 ）. Conclusion： The tumor suppressor gene ARHI is generally expressed in endometrium, and especially higher in ectopic endometrium with endometriosis. ARHI may be involved in the development of endometriosis.