大鼠口服红花水提物和红花黄色素后羟基红花黄色素A的排泄研究

An Analysis of Hydroxysafflor Yellow A Excretion of Rats After Oral Administration of Safflower Extracts and Safflor Yellow

建立简便的高效液相色谱法(HPLC)测定大鼠口服红花水提物和红花黄色素后尿、粪和胆汁中主要活性成分羟基红花黄色素A(HSYA)的含量。色谱柱为Hypersil BDS-C18(250×4．6mm,5μm),流动相为乙腈-0．3%冰醋酸水溶液梯度洗脱,检测波长320nm,葛根素或对羟基苯甲醛为内标。尿、粪和胆汁中的HSYA的日内精密度小于3．1%,日间精密度小于5．8%。提取回收率为79．2%～102．1%。该方法首次研究了大鼠口服红花水提物和红花黄色素后羟基红花黄色素A的排泄情况。

In this study, a simple and reproducible high -performance liquid chromatography （HPLC） method was employed to determine hydroxysafflor yellow A （HSYA） in rat urine, feces and bile, after an oral administration of safflower extracts and safflor yellow. HPLC was performed on a Hypersil BDS -C18 column, with a gradient elution system that is made up of acetonitrile and aqueous acetic acid. Ultraviolet detection was performed at 320 nm, with puerarin and p - Hydroxybenzaldehyde as the internal standard （IS）. The intra- day and inter- day precision were registered less than 3.1% and 5.8% respectively, and the extract recovery ranged between 79.2% - 102.1% in urine, feces and bile. The effort makes the first instance of using HPLC method to work on the excretion rates of HSYA in rat urine, feces and bile, after an oral administration of safflower extract and safflor yellow.