姜黄素对沙土鼠脑缺血再灌注后海马CA1区及CA3区Bcl-2、Bax表达的影响及意义

The effect of curcumin on the expression of apoptosis-related genes Bcl-2 and Bax after cerebral ischemia in hippocampal CA1 and CA3 areas and its significance

目的探讨姜黄素对沙土鼠脑缺血再灌注后凋亡相关基因Bcl-2、Bax在海马CA1区、CA3区表达的影响及意义。方法制作沙土鼠双侧颈总动脉阻断缺血再灌注损伤模型。随机分为假手术组(SH)、脑缺血再灌注组(IR)、姜黄素组(CU)、对照组(SC),每组据再灌注时间点不同又分为1、3、57、d 4个亚组,每组6只动物。在预定时间点行开阔法行为学检查,TUNEL法检测海马CA1区、CA3区细胞凋亡,免疫组织化学染色测定Bcl-2、Bax蛋白在海马CA1区、CA3区的表达变化。结果姜黄素可显著减少沙土鼠探索活动及海马CA1、CA3区凋亡锥体细胞数量(与IR组相比,P<0.01),诱导Bcl-2蛋白及抑制Bax蛋白表达(与IR组相比,P<0.01)。结论姜黄素有脑保护作用,调控凋亡相关基因Bcl-2、Bax蛋白的表达可能是其作用机制之一。

Objective To investigate the expression of apoptosis-related genes Bcl-2 and Bax after cerebral iscbemic reperfusion in hippocampal CA1 and CA3 areas and the effect of curcumin on the expression of Bcl-2 and Bax in gerbils. Methods Gerbils were randomly divided into sham group （SH）, ischemia-reperfusion group （IR）, curcumin group （CU） and solvent control group （SC）. Forebrain ischemia was induced by occlusion of bilateral common carotid arteries. Observations were made in each group 1 d , 3 d , 5 d and 7 d after ischemia. Open field test was used to examine the behavioral change. The apoptotic neurons in hippocampal CA1 region was counted, and the expression of Bcl-2 and Bax in hippocampal CA1 and CA3 areas was detected by SABC immunocytochemical technique. Results The behavioral mark and the number of apoptotic neurons in hippocampal CA1 and CA3 areas were much smaller in CU group than in IR group （P〈0. 01）. The expression of Bcl-2 was greater and the expression of Bax was less in CA1 and CA3 areas in CU group than in IR group （P〈0. 01）. Conclusion Curcumin can protect neurons against cerebral ischemia. It may be one of the protective mechanisms by modulating the expression of apoptosis-related genes Bcl-2 and Bax.