葡萄糖、胰岛素和尿酸对猪近端肾小管上皮细胞株血红素氧合酶-1的影响

Effects of glucose,insulin and uric acid on the expression of hemoglobin oxygenase in pig renal tubule cell line

目的观察不同浓度葡萄糖、胰岛素和尿酸的培养条件下猪近端肾小管上皮细胞株(LLC-PK1)血红素氧合酶(HO-1)活性的改变,以探讨代谢相关因素在细胞水平对肾小管上皮细胞的影响。方法以LLC-PK1为研究对象,观察葡萄糖浓度为5、10、22、33 mmol/L,胰岛素浓度为0、10-9、10-8、10-7mol/L,尿酸浓度为0、0.1、0.2、0.4 mmol/L培养48 h条件下,LLC-PK1细胞HO-1活性的改变。结果0.1 mmol/L0、.2 mmol/L和0.4 mmol/L尿酸刺激48 h后,LLC-PK1的HO-1活性增加,并呈剂量依赖关系,而上述浓度的葡萄糖和胰岛素刺激48 h对LLC-PK1的HO-1活性无影响。结论一定浓度的尿酸可使LLC-PK1的HO-1活性升高,提示尿酸在细胞水平对肾小管上皮细胞的氧化应激过程产生影响。

Objective To investigate the effects of metabolically correlated factors on pig renal tubule in cellular level by observing effects of glucose, insulin and uric acid on activity of haemachrome oxygenase in renal tubular epithelial cells. Methods Pig near renal tubular cell line （LLC-PK1） was selected as experimental object. After stimulating cells respectively with glucose （5, 10, 22, 33 mmol/L）, uric acid （0, 0.1, 0.2, 0.4 mmol/L） ; and insulin （0, 10^-9, 0^-8, 10^-7 mol/L） in different concentrations for 48 hours, the activity of HO-1 within the cells was determined. Results In uric acid group the activity of HO-1 was significantly increased in a concentrationdependent manner,while in glucose and insulin groups, the activity of HO-1 did not change. Conclusion After LLC-PK1 was stimulated by uric acid of different concentrations, HO-1 was induced evidently, which indicates that uric acid might affect oxidative stress in renal tubule cell line.