ICP-AES法测定血硼及儿童血硼质量浓度调查

Determination of blood boron by inductively coupled plasma atomic emission spectrometry and investigation of boron level in children's blood

目的探索电感耦合等离子体原子发射光谱(ICP-AES)法测定血硼的方法,确定南京地区儿童血硼质量浓度正常值范围。方法全血经1 mol/L HNO3处理后用ICP-AES法测定,并用该方法测定南京地区1 032名健康儿童及1 364名就诊儿童血硼。结果方法精密度为1.60%-4.31%,回收率为93.3%-98.9%。1 032名健康儿童全血硼质量浓度为(41.8±16.7)μg/L,1 364名就诊儿童血硼质量浓度为25.1-58.5μg/L(77%)。结论全血经硝酸处理后测定血硼,精密度及回收率均较理想,可以作为测定血硼的参考方法。并初步提出该方法的南京地区儿童血硼参考范围为25.1-58.5μg/L(1岁以下儿童除外)。

Objective To study inductively coupled plasma atomic emission spectrometry （ICP-AES） used to determine blood boron （B）, and to investigate the level of B in blood of children in Nanjing. Methods ICP-AES was applied to determine the level of B in the blood of 1 032 healthy children and 1 364 children patients after using 1 mol/L HNO3 to treat whole blood. Results Precision of this method was 1.60% - 4.31%, and recovery ratio was 93.3 % -98.9%. The level of B in blood of the 1 032 healthy children was （41.8 ± 16.7） μg/L and that in blood of the 1 464 patients was （25.1±58.5） μg/L （77%）. Conclusion Precision and recovery ratio determined by the method are high, so. it can be used as a reference method to detect the level of B in blood. And it preliminarily determines the reference levels of blood B of children in Nanjing as ranging from 25.1-58.5μg/L （except children below 1 year old）.