AQP4参与雌激素对单胺类神经递质的调节作用

AQP4 deficiency alters regulative effects of estrogen on monoamine neurotransmitters

目的:探讨AQP4对雌激素调节神经递质作用的影响。方法:应用雄性AQP4基因敲除型CD1小鼠与野生型CD1小鼠,给予不同剂量雌激素后测定不同脑区中单胺类神经递质的含量。结果:AQP4基因敲除型雄性小鼠纹状体多巴胺(DA)及5-羟色胺(5-HT)、皮层去甲肾上腺素(NE)含量增加;海马NE、下丘脑DA水平下降。同时,给予雌激素后,野生型雄性CD1小鼠纹状体DA、5-HT含量及海马5-HT含量升高,皮层DA、5-HT水平及下丘脑NE、DA水平下降;而雌激素对AQP4敲除型雄性CD1小鼠单胺类递质无显著影响。结论:AQP4敲除可改变雌激素对单胺类递质水平的调节作用,对纹状体内DA水平的影响尤为显著。AQP4参与了雌激素对单胺类神经递质的调节。

AIM： To investigate whether aquaporin-4 （AQP4） participates in the regulative action of estrogen on neurotransmitters （NT）. METHODS： The monoamine neurotransmitters were determined by high performance liquid chromatography （HPLC） in different brain regions of male AQP4-knockout CD1 mice and wild-type CD1 mice after they were intmperitoneally injected with different doses of estmdiol. RESULTS： The contents of DA and 5-HT in the striatum and NE in the cortex of AQP4- knockout CD1 were significantly increased, and the concentrations of NE in the hippocampus and DA in the hypothalamus were decreased. Treatment with estradiol increased the contents of DA and 5-HT in the striatum and that of 5-HT in the hippocampus, decreased the levels of DA and 5-HT in the cerebrum, and also reduced the concentrations of DA and NE in the hypothalamus in wildtype mice. However, these changes were not found in AQP4-knockout mice. CONCLUSION： The deficiency of AQP4 in mice alters the regulative effects of estrogen on , monoamine neurotransmitters, indicating that AQP4 participates in regulating metabolism of neurotransmitters.